QUANTITY: An Isobaric Tag for Quantitative Glycomics

نویسندگان

  • Shuang Yang
  • Meiyao Wang
  • Lijun Chen
  • Bojiao Yin
  • Guoqiang Song
  • Illarion V. Turko
  • Karen W. Phinney
  • Michael J. Betenbaugh
  • Hui Zhang
  • Shuwei Li
چکیده

Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2015